ω-Transaminase from Ochrobactrum anthropi is devoid of substrate and product inhibitions.
نویسندگان
چکیده
ω-Transaminases display complicated inhibitions by ketone products and both enantiomers of amine substrates. Here, we report the first example of ω-transaminase devoid of such inhibitions. Owing to the lack of enzyme inhibitions, the ω-transaminase from Ochrobactrum anthropi enabled efficient kinetic resolution of α-methylbenzylamine (500 mM) even without product removal.
منابع مشابه
Improvement of whole-cell transamination with Saccharomyces cerevisiae using metabolic engineering and cell pre-adaptation
BACKGROUND Whole-cell biocatalysis based on metabolically active baker's yeast with engineered transamination activity can be used to generate molecules carrying a chiral amine moiety. A prerequisite is though to express efficient ω-transaminases and to reach sufficient intracellular precursor levels. RESULTS Herein, the efficiency of three different ω-transaminases originating from Capsicum ...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 79 13 شماره
صفحات -
تاریخ انتشار 2013